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In my limited knowledge of this area, I seem to have the impression that
expression of antibody on the cell surface does not necessarily correspond
to the quantity of antibody secreted from a hybridoma cell. I'd be very
interested to obtain opinions as to whether sorting of these cells based on
high surface expression is a reasonable thing to do for improvement of
antibody production.
Thanks in advance,
Neal
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Neal A. Benson Tel: (904) 392-0008
University of Florida Fax: (904) 392-4693
Department of Pathology Email: nbenson@nervm.nerdc.ufl.edu
Box 100275
Gainesville, FL 32610-0275 USA
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web