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I am currently working with an androgen independent prostate
cancer cell line. Using the Oncor ApopTag kit, I have gotten beautiful
pictures of cells undergoing apoptosis, however, there are a few
phenomenas that I notice that I can't explain- namely:
1. Apoptosis is supposed to be marked by cell shrinkage whereas
my cells (which underwent ionizing irradiation) are markedly larger than
the control cells.
2. In the positive controls and cells that undergo a lot of
apoptosis, I notice a lot of cells have FITC label only on the membrane
creating a "ring" around these cells. Is this indicative of early apoptosis?
(the cells have been fixed)
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web