CD34+: methylcellulose colony assay vs. FACS analysis
Penney Koeppen (Penney_Koeppen@hgsi.com)
Fri, 30 Jun 95 11:20:52 EDT
Has anyone used FACS analysis of liquid cultures of CD34+ cells from cord blood
to reproduce data obtained from parallel methylcellulose colony assays? eg:
Would the number of BFU-E's in methylcellulose correlate with % glycophorin A-
positive cells in a parallel liquid culture; and would the number of CFU-meg's
correlate with % CD41a-positive cells?
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