 |
 |
 |
 |
 |
> I intend to use fluorogenic beta-galactosidase substrate to study gene
> expression in SW480-cells that are stable transfected with the gene encoding
> beta-gal (reporter-gene).....
We have been using the FluoReporter kit from Molecular Probes part
number F-1930 (50 test kit). We find it works pretty well. No fixation is
required and the cells can easily be sorted and even grown. Transfected
cells are usually about a log up from the control or untransfected cells.
You must however keep the sample cold as untransfected cells quite rapidly
convert the substrate at room temperature. We havn't co-labelled with
antibodies but I don't see why this should be a problem.
Good luck
Simon Monard
Cambridge
UK
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web