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I intend to use fluorogenic beta-galactosidase substrate to study gene
expression in SW480-cells that are stable transfected with the gene encoding
beta-gal (reporter-gene). I will analyse beta-gal activity in conjunction
with other cell markers. If it is possible with these substrates, I also
want to sort high and low expressing cells and do a further study of
membrane receptors and intracellular components.
According to Molecular Probes there are various sustrates available: FDG,
carboxy-FDG, dichloro-FDG, chloromethyl-FDG(DetectaGene) and
C12-FDG(ImaGene). But I am not familiar with the use of these.
If you have any experience with these or other beta-gal substrates used in a
similar manner, I will find it very beneficial to have comments, hints,
references etc.
Flow a nice day!
K.Egeberg
Kjartan Egeberg
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web