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The anti-FITC antibodies are supposed to quench the Fluorescein fluorescence.
If you have an anti-FITC-PE then the PE fluorescence should replace the
FITC fluorescence. Haven't done it yet, just read about it.
Debbie Berglund
Microbiology
Montana State University
Bozeman, MT
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web