Howard Shapiro wrote>
>Many years ago Gunther Valet used fluorescein-tagged polyionic
>materials to estimate surface charge; would a similar approach work for
>you? -Howard
>
This should work; except fluorescein is the wrong dye. As Alan Wagonner,
now at Amersham Life Science in Pittsburgh, has pointed out, increasing the
charge on a dye will inhibit stacking and thus, decrease fluorescence quenching.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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