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> With some of the tandem conjugates for FL3, I have to
> compensate 5 to 15% to get them out of FL2 and may see
> spill over into FL1.
But...
Since the spillover of tandems into FL1 is due to unquenched PE emission, as
long as you are doing proper compensation between PE and FITC (FL2 to FL1) as
well as the tandem and PE (FL3 to FL2), then there cannot be any uncorrected
spillover between FL3 and FL1.
mr
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
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Phone: (765)-494-0757;
FAX(765) 494-0517;
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