Membrane polarization
Jan Bly (bly@umsmed.edu)
Mon, 15 Apr 1996 23:10:01 -0500 (CDT)
We are trying to measure membrane polarization by flow cytometry
using the probe TMA-DPH. So far we've had no luck. Is anyone else doing
this? If so, can you tell me which probes you have found most successful
and the specs on your polarization filters. We are using a FACS Vantage
and "hand-me-down" polarizers (no specs). The polarizers we're using are
yellow, and we're wondering if we were just cutting out most of the
TMA-DPH emitted signal.
TIA for any help, Jan Bly
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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, EMAIL
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