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We are interested in knowing if someone has experience using TO-PRO-3 on
chord blood, bone Marrow or peripheral blood studies.
If so, have you used it with other fluorochromes?
Are their any advantages/disadvantages or problems in its use?
Thank you in advance,
Mike and Barb
Stem Cell Research Lab
American Red Cross
Portland Oregon
We are using TO-PRO-3 routinely for DNA content measurements in =
combination with two monoclonal antibodies (indirectly labeled with FITC =
and PE). When we started this type of triple labeling experiments we =
tried to combine FITC, PE and propidium iodide as well. However, using =
20 - 40 microgram/ml of PI, we had to compensate so much that we didn't =
trust the DNA histogram calculations anymore. Now we are using TP3 =
instead. The only disadvantage of the dye is the need for a second =
laser, a He-Ne laser for excitation at 633 nm. We use the standard =
coulter 675 nm BP filter for collection of the TP3 emission.
The technique has been described in a publication in Cytometry, 1994, =
17: 185 - 189.
We have applied the technique mainly to skin research. Examples can be =
found in: Acta Derm Venereol (Stockh) 1995; 75, 381 - 385 and 437 - 441 =
and very recently in Arch Dermatol Res 1996, 288, 203 - 210.
=20
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Piet van Erp
University Hospital Nijmegen
Department of Dermatology
P.O. Box 9101
6500 HB NIJMEGEN /The Netherlands
Voice: +31 (24) 3616409
Fax: +31 (24) 3541184
e-mail: p.vanerp@derma.azn.nl
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web