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We used normal saline (.9% - same stuff they plugged all the hospital
patients into) happily for many years before switching to PBS
(Phosphate Buffered Saline) for our sheath fluid.
As for concentration of NaCl for electrical conductivity for sorting - not
to worry. Water with 1/50th of the concentration of normal saline will
happily conduct your drop charges.
Richard Stovel stovel@darwin.stanford.edu
Herzenberg Laboratory and Stanford Shared FACS Facility
Stanford University, Stanford CA
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web