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Try Mitomycin C to arrest your cells. It should do the trick. Need a protocol? email me direct.
>Hi
>
>All you cell cycle experts out there, can any one suggest ways of blocking
>cell cycle without killing cells. We would like our cells (T-cell lines) to
>remain arrested for several days without dying. We need the cells to be in
>G1, S or G2. We have tried gamma radiation and aphidicoline but the cells
>do not survive to the end of the assay.
>
>Simon Monard
>Aaron Diamond Center
>New York
***********************************************
Roger A. Burger E-mail: Roger@cpd2.usu.edu
Research Immunologist
Center for Persons with Disabilities
Utah State University
Logan, UT 84322-6895
Voice: 801-797-2042
FAX: 801-797-2044
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web