TdT antibody selection
Tom Mchugh (mchugh@pangloss.ucsf.EDU)
Tue, 5 Mar 1996 17:43:22 -0800 (PST)
We currently do TdT determination for leukemia characterization on
cytocentrifuge preps using the rabbit polyclonal anti-TdT. We have
however for years been looking at doing the test by flow cytometry with
monoclonal anti-TdT. There are anecdotal reports, at least, that the
monoclonal anti-TdT misses some ALL's which are positive by the rabbit
anti-TdT. I have recently heard that the mixture of 3 monoclonals to TdT
which has been the popular mixture is not as good as a new single monoclonal
anti-TdT. Does anyone know what is really going on here?
Thanks
Tom McHugh
mchugh@labmed.ucsf.edu
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
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