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Energy transfer from FITC to PE would be expected to increase the
yellow-orange and decrease the green fluorescence signal. The PE signal
would then look undercompensated, while the FITC signal would look
overcompensated. Compensation of the conventional type assumes that there
isn't significant energy transfer; how to compensate when there is is a
philosophical question.
--Howard
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web