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There will be a spectral shift depending on the distance of the
fluorophores. E.g. FITC-Emission generated by an PLA1
alloantibody will be absorbed by an anti-GpIIb/IIIa monoclonal
depending on distance of the epitopes. The method can
be used for the characterization of human anti-platelet
antibodies. We published the method in
J. Immunol. Methods 187: 53-67, 1995.
Gregor Rothe
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Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, F.R. Germany
Tel. +49 (941) 944-6204
Fax +49 (941) 944-6202
Internet: Gregor.Rothe@klinik.uni-regensburg.de
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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