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Purified green fluorescent protein (GFP) absorbs blue light maximally at 395
with a minor peak at 470 and emits at 509 with a shoulder at 540.
For more information see:
1). Green Fluorescent Protein as a marker for gene expression. Chalfie, et al.
Science ( 263) pp 802-5
2) Wavelength mutations and posttranslational autoxidation of green
fluorescent protein. PNAS 91 (26) 12501-4
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web