Re: Green Fluorescent Protein

Zawadzki, Jerome A. (jzawadzki@molecular.princeton.edu)
Fri, 28 Apr 95 14:05:00 PDT

We have GFP expressed in a variety of systems, yeast, slime mold and in a
human cell cell line and use either 476 or 488 to excite and a 510 or
525bp filter for the emission depending on application. Funny things
happens under high and low laser power. Also, a nice paper in Dec PNAS, but
not flow. Have fun. JAZ
----------
From: owner-cyto-sendout
To: Cytometry Mailing List
Subject: Re: Green Fluorescent Protein
Date: Thursday, April 27, 1995 5:44PM

---Kier Watkins wrote:
Someone has asked me about using Green Fluorescent Protein for flow
analysis
of transfected sperm.
What's the excitation/emission?
--- end of quoted material ---

I'm not sure whether the puns are intentional or not.... but I'll pretend I
didn't notice them. There is a paper in Science 263:802(1994) by Chalfie et
al
that shows GFP spectra. Excitation has a peak at 395nm with a shoulder at
470nm and fluorescence has a peak at 509nm with a shoulder at 540nm.

Alice Givan
Englert Cell Analysis Laboaratory
Dartmouth Medical School
Lebanon, NH 03756
USA

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