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>What's the excitation/emission?
The native protein is excited at 396 and 475nm (minor) and has emission at
509nm.
Changing a single amino acid gives rise to an improved form with excitation
at 490 and emission at 510nm (Nature 373 663-664).
I have been trying to get our molecular biologists to try the GFP but they
are a conservative lot and hooked on Glutathione Sulpho-Transferase, which
is a pain to analyse by flow.
Dean R. Hewish, Cell Biologist & Flow Cytometrist.
CSIRO Biomolecular Engineering, 343 Royal Parade, Parkville, 3052,
Victoria Australia.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web