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> I've been looking all around to find a flow cytometric technic to
> assess lipid peroxidation in cells suspension.
Molecular Probes sells a fluorescent reagent named ADIFAB which reports
the concentration in cell cytoplasm of long chain unbound fatty acids
(both saturated and unsaturated). There is the possibility that lipid
peroxidation will affect fatty acid metabolism so as to shift the free
fatty acid concentration -- it may not have been explored. So this
is just an idea to put in the hopper ...
Good luck,
/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web