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I was wondering if anyone out there has any experience in looking at
rabbit fiberblasts and schwann cells? I have an investigator who is
growing cultures of fiberblasts and want to know the percent contamination
due to schwann cells using Flow Cytometry. Can this be done? What do the
light scatters of rabbit fibroblasts and schwann cells look like in the same
culture. My plots have one population large and smooth, and a second very
small and smooth. The small size population almost looks like debris.
Do I have a correct plot of the mixed culture? If the light scatters are
not correct, are there any antibodies that will see rabbit fibroblasts or
schwann cells. Any help is greatly appreciated.
DAVIN B. JUTILA *--------------* SIU SCHOOL OF MEDICINE
FLOW CYTOMETRY FACILITY - 1220 SPRINGFIELD, IL 62702
PHONE: (217)-524-5812 FAX: (217)-524-3227
EMAIL: SM0973%SPRINGB@SIUCVMB.SIU.EDU
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web