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We use a 9 Litre tank on our old FACS II. We modified the tank as per a
short note (Constant Pressure Sheath Tank, M. Wilder & J. Jett) in an old
Los Alamos `Flow Systems Newsletter'.
Sheath Pressure _________________ Sheath Liquid
------------ | _______________
---------- | | |
____|_|__|_|____
|B | | | | |
| | | | | |
|--| |--| |--|<-- Fluid Level
| | | | | |
| | | | | |
| | | | | |
| |A| |
| |
------------
When connected as such, the pressure at point A is constant and independent
of liquid head height.
The pressure difference between A and B is due to the fluid level head
height. Hence by connecting a differential pressure guage between A and B,
one can also have a sheath fluid level indicator.
I haven't actually connected a pressure guage at the nozzle to see how well
this works, but we don't have too much trouble over the period of an
afternoon.
It's a long time since I've thought about the physics behind the above but
it is similar to the way we used to (many moons ago as a student!) connect
up fluid reservoirs, when running columns, to get constant flow.
I can't find the original newsletter but I hope this may be useful.
Leon.
###########################################################################
____
| | Dr. Leon Martin,
/ | _ Dept. of Pathology & Immunology,
| |-------> ( ) Monash University Medical School,
\ | ~ Commercial Rd., Prahran, Victoria,
|____| /| Australia, 3181.
__| |__ ___/ |
|_______|^^^^ --- e-mail: martin@cobra.path.monash.edu.au
| Phone: +61 3 276 2601
| Fax: +61 3 529 8359
--
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web