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>
> I have been asked to test / set up our single cell deposition system for
> sorting single cells into Terasaki plates. Sounds like a challenge to me,
> but can it be done? FACStar Plus, ACDU, macrosort head. Isn't that sorted
> drop going going to make like a giant meteor on earth and blow all to well?
> Is the sorter accurate enough to spit a drop / cell into such a small well /
> volume in the first place? Answers to these and other questions would be
> much appreciated, as well as tips on how to do it... if it can be done!
> Thanks, Claude
>
I have done it on a Vantage with a TSO. It takes a bit of time to adjust
the well differences. Be patient. It is not if the sorter can do it, but
can the operator? Use 10-12 psi sample pressure.
Good luck,
Jim
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web