absolute counts

Dave Novo (novod@muss.CIS.McMaster.CA)
Tue, 25 Feb 1997 19:42:22 -0500 (EST)

Hello Everyone,

I am trying to do absolute counts of bacteria. I have been using a
kit but its about $100 for 100 samples. My problem is that the kit keeps
dissapearing faster than the bottle of vodka I keep on the lab shelf for
days that experiments don't work. I am wondering if anyone has a
cheaper/longer lasting suggestion for absolute counting of cells on the
FACScan..

Thanks for your help.
I will compile the suggestions and post them for future reference.

Dave

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I have nothing to put here that anyone would care about. But since
everyone has one of these, I figured I should too . . . . .
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu