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| Stock Number | Description | Tests | |
| PI-STAIN | DNA Staining Reagent Kit | 20 | |
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The DNA staining kit provides reagents for the isolation and staining of cell nuclei
from fresh tissue fragments or peripheral blood. Nuclei are stained with propidium
iodide (PI) and analyzed by flow cytometry. The resulting histograms can indicate cell
cycle distribution or DNA aneuploidy.
Reference: 1.Vindelov, L.L., et al., Cytometry, 6, 348 (1985).
Store at _20°C. Shipping Conditions: On dry ice.
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| Stock Number | Description | Tests | |
| QC-WIN | QC Windows | 100 | |
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The QC Windows is a unique microbead standard used to achieve a unified setup
protocol for all cytometers. This practice effectively normalizes all fluorescence
intensity data across laboratories and different models of cytometers. The QC
Windows Kit contains a certified blank microbead and a microbead standard for flow
cytometry. The two reagents are uniform in light scatter characteristics and are
provided in separate vials. The certified blank microbead is used to determine the
fluorescent threshold of the instrument (noise level). The microbead standard is
labeled with both FITC and PE and have lot specific target channel values assigned.
These two reagents are used to determine the appropriate PMT voltage setting for
any cytometer. The QC Windows Kit establishes a common Window of Analysis to be
employed across all laboratories and instruments, therefore, permitting the direct
comparison of histogram data.
Store at 2-8°C. Do Not Freeze. Shipping Conditions: Ambient.
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| Stock Number | Description | Tests | |
| QMF-2 | Quantum Fluorescence Kit for MESF units of FITC | 20 | |
| QMF-10 | 100 |
The Quantum Fluorescence Kits for MESF units of FITC use flow cytometry to create
a fluorescence intensity standard which matches the spectral properties of the cell
sample. MESF (Molecules of Equivalent Soluble Fluorochrome) units are a relative
fluorescence intensity unit which compares a stained sample to the fluorescence
intensity of a known solution of equivalent fluorochrome molecules. This is done in a
fluorimeter where both the sample and the standard fluorochrome solutions are
exposed to the same local environment. MESF units are fluorochrome specific. Four
FITC reference standards of microbeads (approximately the size of lymphocytes) are
calibrated against solutions of laser grade FITC in terms of MESF units per
microbead standard. The range of MESF units of FITC is ~10,000 to 500,000. An
unstained microbead population serves as negative control by indicating the
background noise level of the flow cytometer. The handling program ''QuickCal'' is provided on a 3.5² diskette. It analyzes data from practically any flow cytometer using any analysis software. The program contains lot-specific information, namely the MESF units of FITC for each microbead population. The QuickCal Program accepts peak channel data for the microbeads, creates a regression calibration plot and then analyzes the data for the sample cells.
Store at 2-8°C. Do Not Freeze. Shipping Conditions: On wet ice. Please refer to ordering information for details.
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| Stock Number | Description | Tests | |
| QMP-2 | Quantum Fluorescence Kit for MESF units of PE | 20 | |
| QMP-10 | 100 |
The Quantum Fluorescence Kits for MESF units of PE (R-phycoerythrin) use flow
cytometry to create a fluorescence intensity standard which matches the spectral
properties of the cell sample. MESF (Molecules of Equivalent Soluble Fluorochrome)
units are a relative fluorescence intensity unit which compares a stained sample to
the fluorescence intensity of a known solution of equivalent fluorochrome molecules.
This is done in a fluorimeter where both the sample and the standard fluorochrome
solutions are exposed to the same local environment. MESF units are fluorochrome
specific. Four PE reference standards of microbeads (approximately the size of
lymphocytes) are calibrated against solutions of laser grade PE in terms of MESF
units per microbead standard. The range of MESF units of PE is ~10,000 to 500,000.
An unstained microbead population serves as negative control by indicating the
background noise level of the flow cytometer. The handling program ''QuickCal'' is provided on a 3.5² diskette. It analyzes data from practically any flow cytometer using any analysis software. The program contains lot-specific information, namely the MESF units of FITC for each microbead population. The QuickCal Program accepts peak channel data for the microbeads, creates a regression calibration plot and then analyzes the data for the sample cells.
Store at 2-8°C. Do Not Freeze. Shipping Conditions: On wet ice. Please refer to ordering information for details.
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| Stock Number | Description | Tests | |
| QMF-P-2 | Quantum Fluorescence Kit for MESF units of FITC and PE | 20 | |
| QMF-P-10 | 100 | ||
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For QMF-P-2, One of each QMF-2 and QMP-2 Kit is shipped. For QMF/P-10, One of each QMF-10 and QMP-10 Kit is shipped.
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| Stock Number | Description | Tests | |
| QSC-20 | Quantum Simply Cellular Microbead Kits | 20 | |
| QSC-100 | 100 |
The Quantum Simply Cellular microbeads use flow cytometry to measure the quantity
of cell surface antigen molecules in Antibody Binding Capacity (ABC) units. ABC is
the number of antibodies bound per cell or microbead. These microbeads are
designed for direct or indirect assays which use fluorescent labeled mouse primary
antibody or fluorescent-labeled anti-mouse secondary antibody. Four populations of
microbeads (approximately the size of lymphocytes) are produced to bind defined,
incremental amounts of mouse immunoglobulin. A non-binding microbead population
serves as negative control by indicating the detection limit of the cytometer.
Microbeads bind staining reagents through covalently-bound, polyclonal anti-mouse
immunoglobulins. This antibody has equivalent reactivity to each mouse isotype
(IgG1, IgG2a and IgG2b). The data handling program ''QuickCal'' is provided on a 3.5 inch diskette for IBM and Macintosh. It analyzes data from practically any flow cytometer using any analysis software. The program contains lot-specific information, namely the ABC assignments for each microbead population. The QuickCal Program accepts peak channel data for the microbeads, creates a regression calibration plot and then analyzes the data for the sample cells.
Store at 2-8°C. Do Not Freeze. Shipping Conditions: On wet ice. Please refer to ordering information for details.
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