Mouse Isotype Controls for Flow Cytometric Analysis

The specificity of staining by monoclonal antibodies to mouse CD antigens should be verified by establishing the non-specific reagent binding to the target cell population. It is recommended that a concentration and isotype matched mouse myeloma immunoglobulin be included as a negative control for each monoclonal antibody reagent used in the immunoassay procedure.

Sigma Mouse Isotype Controls are mouse myeloma proteins tested for use as controls in flow cytometry.

Sigma Mouse Isotype Controls are supplied to match the concentration of Sigma purified and/or conjugated monoclonal antibodies to CD antigens. These products are liquid, 100 mg/vial, in 0.5 ml of 0.01 M phosphate buffered saline (PBS), pH 7.4, containing 1% BSA and 0.1% sodium azide as preservative. DUAL-TAG controls contain 100 mg of each conjugate, or 200 mg/vial in 1 ml.

Mouse Isotype Controls conjugated to FITC, R-Phycoerythrin (PE) or Quantum Red^TM may be used as controls for monoclonal antibodies to CD antigens in direct immunofluorescence procedures. Each isotype control is prepared with a F:P (fluorochrome:protein) molar ratio in the range similar to the specific monoclonal antibody conjugates offered by Sigma.

Store at 2-8°C. Shipping Conditions: Ambient.

U.S. Patent No. 4,859,582; 4,542,104

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu