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CytoStain™ Cell Fixation and Permeabilization Kits

Cytofix/Cytoperm™ Kit

Catalog #2075KK

This kit enables the one step fixation and permeabilization of cells necessary prior to the staining of intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. This kit provides two reagents: Cytofix/Cytoperm™ fixation/permeabilization solution and Perm/Wash™ buffer. After fixation/permeabilization using the Cytofix/Cytoperm™ solution, the Perm/Wash™ buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining. It is important that the Perm/Wash™ buffer be used for dilution of anti-cytokine antibodies, rather than a standard staining buffer, in order to maintain cells in a permeabilized state for intracellular staining.

Kit components:

Cytofix/Cytoperm Plus™ Kit (with GolgiStop™)

Catalog #2076KK

In addition to the fixation/permeabilization and diluent/wash solutions included in the Cytofix/Cytoperm™ kit, the Cytofix/Cytoperm Plus™ kit provides GolgiStop™, a protein transport inhibitor for use during the last few hours of cell culture. Addition of GolgiStop™ to cell activation cultures will block intracellular transport processes, thereby resulting in the accumulation of most cytokine proteins in the Golgi complex and enhancing cytokine staining signal.

Kit components:

For Research Use Only. Not For Diagnostic or Therapeutic Use.

PharMingen supplies anti-cytokine antibodies directly conjugated with FITC or PE for intracellular analysis in mouse and human systems. Available specificities include:
Mouse Human
IL-2 IL-1a
IL-3 IL-2
IL-4 IL-4
IL-5 IL-5
IL-6 IL-6
IL-10 IL-10
IL-12 (p40/p70) IL-12 (p40/p70)
IFN-gamma GM-CSF
MCP-1 IFN-gamma
TNF-alpha MCP-1

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu