Map 5-CellProbe Reagents

DCFH, PMA-Oxidative Burst

Part Number: 7547078

The conversion of nonfluorescent dichlorofluorescin diacetate (DCFH-DA) to the highly fluorescent compound 2',7'-dichlorofluorescein (DCF) can be used to monitor the oxidative burst in polymorphonuclear leukocytes. The enzymes responsible for the oxidative burst are rapidly activated in stimulated neutrophils. DCFH,PMA¨Oxidative Burst contains the compound phorbol myristate acetate (PMA), an analogue of the cellular signaling molecule diacylglycerol (DAG). Therefore, the presence of PMA stimulates processes mediated by DAG, including the oxidative burst.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web EMAIL