P-Glycoprotein (mdr 1 gene product)

Clone & Isotype	Conjugate	Size	Format			Part Number
UIC2	Purified	0.1 mg	Freeze-dried			IM1873
IgG2a (mouse)	Purified	100 tests	Liquid			IM1864

The UIC2 antibody reacts with an external epitope of the human P-glycoprotein (P-gp), a 170-180 kDa Multi-Drug Resistance (MDR) gene product. This protein carries out ATP-dependent efflux of structurally diverse lipophilic compounds, including many anti-cancer chemotherapy agents. In hematopoietic cells, the mdr1 gene product is expressed, but at relatively low levels. P-gp is expressed in specialized epithelial cells with secretory or excretory functions. In the liver, P-gp is found on the biliary surface of hepatocytes and small biliary ductules; in the pancreas, on the laminal surface of epithelial cells of small ductules and, in the kidney, on the brush border of the proximal tubules. Additional sites of expression include the gastrointestinal tract, endothelial cells of the brain and the adrenal glands. UIC2 antibody inhibits Pgp-mediated efflux and potentiates the cytotoxic action of P-gp transported drugs in MDR cells. It is suitable for flow cytometry, immunoblotting and immunohistochemistry on cryostat or paraffin embedded tissue sections.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu