PCNA

Clone & Isotype	Conjugate	Size	Format	Line		Part Number
19F4	Purified	100 tests	Freeze-dried	COULTER CLONE		6604542
IgG1 (mouse)	FITC	0.5 mL	Liquid	COULTER		6607002
19A2	Purified	100 tests	Freeze-dried	COULTER CLONE		6604541
IgG1 (mouse)
5A10	Purified	50 µg	Freeze-dried			IM1510
IgG1 (mouse)

Proliferating Cell Nuclear Antigen (PCNA), formerly called cyclin, is a 36 kDa proliferation-associated antigen. It is an intracellular polypeptide antigen that is found in both normal and transformed cells. The level of PCNA synthesis correlates directly with rates of cellular proliferation and DNA synthesis during the cell cycle. Elevated levels of PCNA appear in the nucleus during late G1 phase immediately prior to the onset of DNA synthesis, become maximal during S phase and decline again during G2 and M phases. PCNA is identified as the auxiliary protein to DNA polymerase delta and is tightly associated with the sites of DNA replication. 5A10, 19F4 and 19A2 antibodies recognize human PCNA and are suitable for flow cytometry, immunoblotting and immunohistochemistry on cryostat or paraffin embedded tissue sections. 19F4 antibody was evaluated during the Fifth International Workshop on Human Leukocyte Differentiation Antigens.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu