Map 4-Antibodies

Bromodeoxyuridine (BrdU)

Clone & Isotype Conjugate Size Format Part Number

2B1 Purified 0.1 mg Freeze-dried IM1517
IgG1 (mouse)


Bromodeoxyuridine (BrdU) is a thymidine analog and is incorporated into DNA specifically during the S-phase of the cell cycle. The 2B1 monoclonal antibody recognizes bromodeoxyuridine incorporated into nuclei. It reacts with iododeoxyuridine and bromouridine, but does not react with thymidine. This antibody has been used in research studies of DNA replication, DNA synthesis measured by flow cytometry, kinetic analysis of cell cycle and human tumor proliferation. 2B1 antibody is also suitable for immunohistochemistry on both frozen and paraffin-embedded tissue sections.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu