Map 4-Antibodies

Apoptosis MEBSTAIN Kit

Clone & Isotype Conjugate Size Format Line Part Number
Kit Avidin-FITC 40-66 tests IM1946

In cells in which apoptosis occurs, the chromatin DNA is cut by endonuclease at DNA-linked sites between nucleosomes resulting in a Number of multimers of nucleosomal DNA units in the nuclei.In the TUNEL method (TdT-mediated dUTP-biotin Nick End Labelling method developed by Gavrieli et al. (1992, J.Cell.Biol., vol. 119, 493-501)), 3'-OH DNA ends generated by DNA fragmentation is nick end labelled with biotinylated dUTP, mediated by terminal deoxynucleotidyl transferase (TdT), and then stained using avidin-conjugated FITC to allow specific staining. The MEBSTAIN Apoptosis kit is an apoptosis detection kit based on the TUNEL method.It is suitable for histochemistry (both frozen and paraffin-embedded tissue sections), cytospin preparations, and flow cytometry.
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu