CD95

FAS

Clone & Isotype	Conjugate	Size	Format	Line		Part Number
7C11	RD1	0.5 mL	Liquid	COULTER		6607003
IgM (mouse)
* CH11	Purified	500 µg/mL	Liquid (100 µL)			IM1504
IgM (mouse)
* UB2	Purified	100 µg	Freeze-dried			IM1505
IgG1 (mouse)	FITC	50 tests	Liquid (1mL)			IM1506
	PE	50 tests	Liquid (1mL)			IM1739
ZB4	Purified	100 µg	Freeze-dried			IM1737
IgG1 (mouse)

The CD95 (Fas or APO-1) antigen is a 40-50 kDa transmembrane glycoprotein. It belongs to the Nerve Growth Factor Receptor/ Tumor Necrosis Factor (NGFR/TNF) superfamily and contains three cysteine-rich repeats. This cell surface molecule mediates apoptosis (programmed cell death). CD95 antigen is expressed on a substantial proportion of peripheral CD4+ cells, CD8+ cells and B cells but on a minor proportion of Natural Killer (NK) cells. It is not found on platelets or erythrocytes, but is expressed variably on granulocytes and monocytes. CD95 is strongly up-regulated on activated T cells, B cells, NK cells and thymocytes. Moreover it is widely expressed on cell lines of T, B, NK and myeloid lineage. CH-11 antibody induces apoptosis of some CD95 expressing cell lines, in vitro. ZB4 antibody inhibits apoptosis induced by CH-11 antibody. CH-11 and 7C11 antibodies were evaluated during the Fifth International Workshop on Human Leukocyte Differentiation Antigens.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu