CD45

LEUCOCYTE COMMON ANTIGEN (LCA)

Clone & Isotype	Conjugate	Size	Format	Line		Part Number
KC56 (T-200)	FITC	50 tests	Liquid	CYTO-STAT		6604104
IgG1 (mouse)				CYTO-STAT
Other mAbs

The CD45 molecule family is comprised of at least 5 isoforms, ranging from 180 to 220 kDa. They are generated by alternative splicing combinations of three exons (A, B, and C) of the genomic sequence. The non-restricted CD45 antigen, Leucocyte Common Antigen (LCA), consists of an extracellular sequence, proximal to the membrane, that is the common part of the structure of all CD45 isoforms. All the monoclonal antibodies that belong to the CD45 cluster react with this part of the antigen and are able to recognize all CD45 isoforms. These isoforms have extracytoplasmic sequences ranging from 391 to 552 amino acids long, with numerous N-linked carbohydrate attachment sites. The cytoplasmic part contains two phospho-tyrosine-phosphatase domains. The non-restricted CD45 epitope is present on the surface of all human leucocytes, lymphocytes, monocytes and granulocytes by order of decreasing levels of expression. It is absent from erythrocytes and platelets. It is lost during maturation of erythroid cells in the bone marrow. CD45 antibodies react with leucocyte progenitors in bone marrow. The T-200 antibody was characterized during the Fifth International Workshop on Human Leukocyte Differentiation Antigens.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu