OptiClone^® Products

• Ready to use for flow cytometric analysis.
• Available in one, two and three color configurations for direct immunofluorescent staining.
• Specially formulated for use in a no-wash procedure, using OptiLyse lysing reagent.
• Provided as 50 tests per vial at 20 µL per test.

COMPONENTS AND STORAGE

Antibodies are supplied in phosphate-buffered saline, containing 0.1% sodium azide and 2 mg/ml of bovine serum albumin, unless otherwise specified. The expiration date is indicated on the label. Storage is at 2-8°C, in the dark, without freezing.

FORMAT

Triple-color	FITC/PE/PE-Cy5	50 tests	1 mL
Dual-color	FITC/PE	50 tests	1 mL

FLUOROCHROMES

	Excitation	Emission
FITC (fluorescein isothiocyanate)	468-505 nm	504-541 nm
PE (phycoerythrin)	486-575 nm	568-590 nm
PE-Cy5 (phycoerythrin-cyanin 5)	486-580 nm	660-680 nm

OptiLyse® Lysing Reagent

• Available in two formulations:

OptiLyse C specially formulated for Coulter cytometers.
OptiLyse B specially formulated for B-D and Ortho cytometers

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© Coulter International Corporation, 1996. All rights reserved.
Copyrights and Trademarks

Products are available "For Research use only. Not For Use in Diagnostic Procedures" (RUO). Unless indicated "For In Vitro Diagnostic Use" (IVD).

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu