COULTER GLYCOPHORIN A-RD1

COULTER™ GLYCOPHORIN A-RD1

COULTER GLYCOPHORIN A-RD1 is a monoclonal antibody specific for human red blood cells and erythroid precursor cells. COULTER GLYCOPHORIN A-RD1 is used directly from the vial and is for use in flow cytometric methods. COULTER GLYCOPHORIN A-RD1 is available only for fundamental research and is not for clinical diagnostic use.

Single Parameter Histogram (GLYCOPHORIN A-RD1) A human blood sample was prepared by fixation of the red blood cells. After staining with COULTER GLYCOPHORIN A-RD1, then gating on the sample population of 107 red cells, all red blood cells were recovered as glycophorin A positive (>99%). An isotypic control was analyzed per standard flow cytometric procedures. This assay demonstrates the reactivity of the monoclonal antibody in a known quantity of cells.

Example Research Areas: Detection/isolation of fetal cells Simpson, J.L. & Elias, S. JAMA, 1993, 270(19):2357. Price, J.O. et al. Am J Obstet Gynecol, 1991, 165(6):1731. Identification of erythroid cell lines Braakman, E. et al. Cell Immunol, 1992, 143(1):97. Ligand-induced signal transduction studies Chasis, J.A. & Mohandas, N. Blood, 1992, 80(8):1869. Evaluation of skin discolorations due to bruises Kibayshi, K. et al. Forensic Sci Int, 1993,61(2-3):111.

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Example Histogram

Example of a COULTER GLYCOPHORIN A-RD1 stained erythroleukemia cell line (K562 cells). The histograms represent a light scatter plot, single color isotypic control and single color glycophorin A positive cells.

Molecular Orientation Glycophorin A is a mammalian transmembrane protein. Glycophorin A is one of two main integral membrane proteins that attach to the plasma membrane. The external regions of glycophorin A are heavily glycosylated with O-linked and N-linked carbohydrate chains. Carbohydrate constitutes 64% of the total weight of glycophorin A. Cell Type COULTER GLYCOPHORIN A-RD1 is a pan red blood cell marker; it does not cross react with white blood cells. Ontogeny COULTER GLYCOPHORIN A-RD1 detects all stages of red blood cell maturity.

COULTER (TM) GLYCOPHORIN A-RD1 Specificity The glycophorin A antigen is a sialoglycoprotein. It is present on human erythroid precursor cells through mature red blood cells. It is also present on other mammalian red blood cells. Coulter's red blood cell marker, GLYCOPHORIN A, does not cross react with glycophorin B or C. Product Description COULTER GLYCOPHORIN A-RD1 is in liquid form and is directly conjugated. Concentration: 1µg/10 µL Vial Contents: 0.5 mL Isotype: IgG1 Part Number: 6607000 Chromosome 4 Glycophorin A is located on human Chromosome 4, loci q28-q31.

For Research Use Only. Not for Use in Diagnostic Procedures.

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© Coulter International Corporation, 1996. All rights reserved. Copyrights and Trademarks

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu