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APPLICATION USING FIX & PERM®

AN-DER-GRUB Bioresearch (Austria) has pioneered the development of applications for intracellular antigen detection by flow cytometry. FIX & PERM®, the fixation and permeabilization reagent system developed and manufactured by AN-DER-GRUB and marketed by CALTAG Laboratories, has effectively taken the guesswork out of intracellular flow cytometry. Since the introduction of this unique reagent system, the number of proven intracellular staining applications has grown rapidly. Among the applications identified to date are the following:

LYSOSOMAL PROTEINS

Elastase, Lactoferrin, Lysozyme, Myeloperoxidase, Proteinase-3, CD68

CYTOPLASMIC CD MOLECULES

CD3, CD13, CD22, CD62P, CD63, CD79a

NUCLEAR PROLIFERATION MARKERS

Ki-67, PCNA

NUCLEAR ENZYMES

TdT

ONCOPROTEINS

Bcl-2, c-myc, p53

HIV ANTIGENS

P24

CYTOKINES/CHEMOKINES

Human: IFN-gamma, TNF-alpha, IL1-B, IL-2, IL-4, IL-10, Rantes

Murine: IFN-gamma, TNF-alpha, IL-2, IL-4, IL-5, IL-10

IMMUNOGLOBULINS

Human: IgA, IgG, IgD, IgM, kappa, lambda

Murine: IgG, IgM

OTHER MOLECULES

MHC Class II, Phosphotyrosine, Thrombospondin, Cyclins, Transfected Cells, MDR (Multi-Drug Resistant)

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu