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The PerCP beads allow compensation to be consistent, convenient and
easy (especially if you have the newest FACSComp on a FACStation), but
can't solve the FL1 overlap.
Dennis
*****************************************************************************
* Dennis J. Young Voice : (619)
822-0407 * * Flow Cytometry Core Facility FAX :
(619) 822-0412 * * University of California, San Diego USA
e-mail: djyoung@ucsd.edu *
*****************************************************************************
______________________________ Reply Separator
_________________________________ Subject: PerCP Author:
zinetti@acpub.duke.edu at @UCSD Date: 5/9/96 4:55 PM
Paul J Jansen here,
In the past we have used cychrome (??spell??) as a "third"
color on our one laser Facscan. The problem we had with it was
difficult to compensate between FL1 and FL3. We are considering using
PerCP now and I would like to know if anyone has experience with it.
BD has promised to throw in calibrite beads capable of doing the three
color compensation. Comments? Questions? Suggestions? Thanx Paul J
Jansen zinetti@acpub.duke.edu http://www.duke.edu/~zinetti DUMC
Immunology http://www.duke.edu/~zinetti/Immunology/immuno1.html
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