TUNEL Vagary

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• Next message: Ray Hicks: "Re: Apoptosis"
• Previous message: T. Vincent Shankey: "Apoptosis"

We've been using the FITC-dUTP variant of the TUNEL assay (paraformaldehyde
fixed cells stored in PBS) to study granzyme/perforin mediated apoptosis.
We've recently noted an unexpectedly high percentage of cells being stained
in our negative control populations (yielding a bi-modal populations).

There are only two recent changes:
1) a new lot of 4% paraformaldehyde
2) repeated freeze-thaw cycles of the FITC-dUTP stock.

any help would be greatly appreciated

Thanks in advance

Chris Froelich

• Next message: Ray Hicks: "Re: Apoptosis"
• Previous message: T. Vincent Shankey: "Apoptosis"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu