Re: apoptosis, antibodies against CD95/fas/APO-1

Andreas.Christaras (
Wed, 20 Mar 1996 15:26:04 +0000

On Wed, 13. Mar 1996, Robert Nguyen posted ... :

!> Does anyone know of a good antibody against human Fas (CD 95)? I
!> am using it for Western Blotting purposes.

Following antibodies against human (?) fas-ag (CD95) are commercially
available in
Germany (and world wide, I think) as of 20. Mar. 1996. This list tried but
not accomplished to be comprehensive. Further additions/updates/improvements
are hereby welcomed.

clone class feature form react quant. price suppl. methods
CH-11 IgM induces P H MC-060 50 =B5g 280$ Kam. WB,FC
UB2 IgG1 none P,F,PE H MC-063 100 =B5L 290$ Kam. FC,IHC
ZB4 IgG1 blocks P H MC-072 100 =B5L 260$ Kam. WB,IF,IHC,FC
SM1/1 IgG2a induces P H ??? 50 =B5g 260$ Kam. FC
SM1/17 IgG2b none P H BMS139 1 mL Kam. FC,IF
SM1/23 IgG2b blocks P H ??? 50 =B5g 195$ Kam. FC
CH-11 IgM induces P H 1504 50 =B5g Imm. WB,FC
UB2 IgG1 none P,F,PE H 1505 100 =B5g Imm. FC,IHC
ZB4 IgG1 blocks P H 1739 100 =B5g Imm. WB,IF,IHC,FC
??? IgG1 ??? P H R M F22120 Tlab WB,IF,IP,FC?
Ab-2 IgG1k ??? P H AM01 100 =B5g O/C IF,FC?
Ab-3 IgG2ak ??? P H AM02 100 =B5g O/C IF,FC?
Ab-1 poly WB P H R M PC69 100 =B5g O/C WB
SM1/1 IgG2a induces apopt. P H BMS138 1 mL BMS FC,IF
SM1/17 IgG2b none P H BMS139 1 mL BMS FC,IF
SM1/23 IgG2b blocks apopto. P H BMS140 1 mL BMS FC,IF
??? ??? ??? P H ??? ??? Phar ???

antibodies against fas-ligand (H), remarks see above
clone class feature form react quant. price suppl. methods
33 IgG1 (3) P H F33070 50/150=B5g Tlab FC,IF,WB
Ab-1 poly WB P H PC78 100 =B5g O/C WB
Ab-2 IgG1k WB P H (1) AM12 100 =B5g O/C WB,FC?

abbreviations used:
FC flow cytometry; IF immunofluorescence, WB western blotting, IHC
immunohistochemistry, IP immunoprecipitation, P purified, F FITC-conjugated,=
phycoerythrin-conjugated, H human, M mouse, R rat

(1) According to information I recieved by product manager of the German
distributor of
Calbiochem/Oncogene apoptosis product series, this monoclonal antibody
against the fas-
ligand is specific for the murine protein and N O T for the human. It is
raised against the murine aa-sequence 261-277 which resides within the
cytoplasmic domain.
Interestingly, the catalogue indicates human reactivity on an other page.
Nevertheless, mAb offered by Transduction Laboratories against the human
fas-ligand was also raised against this approximate region of human origin.
This antibody is defined and offered as human-reactive. By the way,
according to my knowledge the mAb established by Nagata-S and co-workers
(Fas-Ligand in human serum, Nature-Med. 2(3), March 1996) is not comercially
available yet.

(2) If PharMingen distributes/manufactures an antibody directed against
human fas-antigen or fas-ligand is not known to me by certainty. You will
have to check this out by phone call or by WWW accession to PharMingen site
on the web indicated below.

(3) Transduction Laboratories anti-human Fas-ligand has an epitope within
the cytoplasmic domain, as checked by GenBank Fas-Ligand sequence U08137
submitted by Mark Alderson from Immunex at Seattle and as confirmed by
German sales representative.

NOTES: Prices are indicated only if information about US dollar pricing was
available to me. Some companies provided me a price list in Deutschmarks,
but because most of fellow cytometrists are not residing wihtin Germany, you
will have to inquire for prices not indicated in aforementioned listings,
All information is wheter acquired by phone calls to companies and/or
distributors or is taken out of catalogues available to me. No liability or
responsibility can be taken/granted for the information given, thogh I tried
hard to be precise as I could be.

Commerical provides/suppliers of antibodies mentioned above are:
Kamiya Biomedical, PO Box 6067, Thousand Oaks, CA 91359, USA, 805-374-1380,
FAX 805-374-1385; effective April 1st, 1996: 910 Industry Drive,
Tukwila, WA 98188, USA, 206-575-8068, FAX 206-575-8094
Immunotech - a Coulter Company -> inquire: Coulter Corp., Hileah, FL, USA
Transduction Laboratories, 2134 Nicholasville Rd., Suite 18; Lexington, KY
40503,606-277-1399, 1-800-227-4063, Fax 1-800-276-2251 (order), tech.
assist.1-606-278-7836, WWW
Oncogene/Calbiochem apoptosis product series
Oncogene Inc., 80 Rogers St., Cambridge, MA 02142, USA, fon 617-492-7289 or
800-662-2616, fax 617-492-8438 or 800-828-4871, E-mail,
WWW (test phase, ineffective prior April
30th, 1996)
Calbiochem International, 84 Rogers St., Cambridge, MA 02142, fon=
or 619-450-9600 or 800-662-2616 or 800-854-3417, fax 800-828-4781 or
617-577-8015, WWW (test phase, ineffective prior
April 30th, 1996)
PharMingen, Inc., WWW:
Bender MedSystems, Dr. Boehringer Gasse 5-11, A-1121 Vienna, Austria, fon
+43-1-80105-378, fax +43-1-80105-477

I hope this list will serve resolving some reagent problems on this very
interesting and new but somewhat "chaotic" field of research. If further
questions arise, do not hesitate to contact me as indicated below.

<><><><><><><><><><><><><><><><><><><><><><>KKKKK KKKKK MMMMM
Andreas Christaras KKKKK KKKKK MMMMMM
Heinrich-Heine University Duesseldorf KKKKK KKKKK MMMMMMM
Dept of pediatric hematology & oncology KKKKKKKKK MMMM MMM MMM
Lab of experimental hematology (KMT-Labor) KKKKKKKK MMMM MMMMM
Geb. 14.82 (Bldg.) KKKKKKKKK MMMM MMM
Moorenstrasse 5 40225 Duesseldorf GERMANY KKKKK KKKKK MMMM M
NOTE:due to the lack of a direct internet KKKKK KKKKK MMMM
access, replying to e-mail may unfortuna- KKKKK KKKKK MMMM
tely take some days. =20
FON : +49-211-81-16103
FAX : +49-211-81-16206 or -16191 B L O O D I S O U R O B S

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