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>I am forwarding a request from a coworker regarding getting a GOOD positive
>control to work in our TUNEL/apoptosis assay. Which nuclease have you
>used (company, ph#, etc) with the best results.
>Thanks again!
>Dan Smith
>San Diego
>e-mail: djs@allp.com (or through the users group)
> ----------
>We have been unable to get the positive control to work in her TUNEL
>protocols. The control involves treating the permeabilized cells with a
>nuclease to nick the DNA and render it TUNEL positive. It must be a problem
>with the enzyme itself since we are registering what we interpret as TUNEL
>positive cells in the treated samples. Could you submit a question to the
>internet group concerning what nuclease and vendor might help us solve this
>problem. We could just start ordering different ones from different places
>but this may give us a more reasonable approach. Long live the NET!
-- ============================================================================== Thomas Delohery | Internet: t-delohery@ski.mskcc.org Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019 1275 York Ave. Box 98 | New York, NY 10021 | ==============================================================================
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