Visiting with the Queen at the Equine Osteochondrosis meetings
Surfing in Mexico with Drs. David Hankey and Gethin Thomas
1. Immunophenotyping of chondrocytes from normal and arthritic cartilage
Specifically we are addressing the development of a comprehensive library of
the cell surface antigens present on chondrocytes collected from healthy and
diseased tissue through the use of immunofluorescent confocal microscopy and
flow cytometry.
2. Basal and stimulated production of reactive oxygen species in situ by
articular cartilage chondrocytes
Inappropriate or pathologic formation of reactive oxygen species has been
implicated in the destruction of a wide variety of extracellular matrices. We are
specifically interested in the relationship the chondrocyte shares with the 3-
dimensional environment of the cartilage and whether alteration of this
environment due to age and disease promote increased production of reactive
oxygen products and enhance subsequent degeneration of both the chondrocyte
and the cartilage.
3. Localization and analysis of estrogen receptors on articular cartilage
chondrocytes
We are looking at the presence of estrogen receptors on articular cartilage
chondrocytes with specific emphasis on age related changes in number and
activity of these receptors and the role these changes may have in predisposing
individuals to degenerative joint diseases.
4. Apoptosis in articular and epiphyseal cartilage chondrocytes
We are specifically interested in understanding if chondrocytes undergo
apoptosis; if so, is the method of apoptosis similiar to that of other cell types and
does age of the individual and pre-existing orthopedic pathologies affect the
apoptotic process.
I would be happy to hear comments, suggestions or criticisms from anyone with an
interest in cartilage, chondrocytes, degenerative joint diseases in humans or animals,
from University of Kentucky basketball fans, dinosaur enthusiasts, or simply interesting
people. I can be reached by E-mail at Jim@flowcyt.cyto.purdue.edu
Curriculum Vitae
James R. Whitfield
Current Address:
P.O. Box 72
Montmorenci, IN 47926-0072
PH: (317)-583-2911
Permanent Address:
53 Nimhan Circle
Carmel, NY 10512
PH: (914)-225-6067
Biographical Information:
DOB 07/20/63
Business Address:
Department of Basic Medical Sciences
Purdue University
Cytometry Laboratory
School of Veterinary Medicine
B050 Hansen Hall
West Lafayette, IN 47907-1515
PH: (317)-494-0757, FAX: (317)-494-0517
Send an email message now to Jim Whitfield
Jim@flowcyt.cyto.purdue.edu
Educational Experience:
10/01/94 - Present
Research Associate
Department of Basic Medical Sciences
School of Veterinary Medicine
02/01/94 - 09/31/94
United States Public Health Service
Post-Doctoral Fellow
Department of Medicine and Biology
University of California at San Diego
1990-1994 Ph.D. awarded 01/21/94
Ph.D. Program, University of Kentucky
Department of Veterinary Science
(Anatomy and Biomechanics)
The Maxwell H. Gluck Equine Research
Center
Dissertation Title: The histology, ultrastructure and phagocytic capabilities of
synoviocytes of normal and Freunds' complete adjuvant treated carpometacarpal and
metacarpophalangeal joints of the horse
1987 - 1990
M.S. Program, University of Kentucky
Department of Veterinary Science
(Anatomy and Biomechanics)
The Maxwell H. Gluck Equine Research center
Thesis Title: An investigation into the use of computed tomography, strain gauges and computer aided design and engineering on the distal equine limb
1983 - 1987
B.S. Program, Delaware Valley College
Department of Animal Science (Nutrition)
1977 - 1981
New York State Honors/Regents Diploma
John F. Kennedy High School
Refereed Publications:
Whitfield, J.R. and D.H. Leach. A computer enhanced computed tomographic,
angiographic and radiographic analysis of normal tarsal joint vasculature of the horse.
Veterinary Radiology. (accepted for publication)
Manuscripts in Preparation:
Whitfield, J.R. and D.H. Leach. The relationship of cartilage canal formation and necrosis and the formation of osteonecrosis dissecans (OCD) in the horse.
Whitfield, J.R. and D.H. Leach. The ultrastructure and phagocytic capabilities of synoviocytes of the metacarpalphalangeal joint: A scanning and electron microscopic study.
Whitfield, J.R. and K.B. Poonacha. Morphometry and histology of the cervical vertebral process in yearling equines with and without cervical vertebral instability (Wobbler Syndrome).
Abstracts in Proceedings:
Whitfield, J.R., K.N. Thompson and J.R. Ronney. (1989) A non-invasive technique for
the measurement of hoof wall deformation. 11th equine nutrition and physiology
symposium, Oklahoma State University. pp. 15-16.
Whitfield, J.R., K.N. Thompson and J.R. Rooney. (1991) The use of computed tomography and computer reconstructions in the study of equine musculoskeletal biomechanics. 18th annual conference of the Veterinary Orthopedic Research Society, Snowmass, Colorado. p. 10.
Whitfield, J.R. and D.H. Leach. (1992) A computer enhanced angiographic and microradiographic analysis of normal and OCD-altered tarsal joint vasculature. 1st International Conference on Equine Osteochondrosis, Cambridge, England. p. 46.
Whitfield, J.R., R.E. Errington and J.P. Robinson. Basal and stimulated production of reactive oxygen species in situ by articular cartilage chondrocytes. To be presented at: International Society of Analytical Cytology, Rimini, Italy. April 11th - 15th, 1996.
Whitfield, J.R. and J.P. Robinson. Apoptosis in articular and epiphyseal cartilage chondrocytes. To be presented at: International Society of analytical Cytology, Rimini, Italy. April 11th - 15th, 1996.
Workshops Attended:
Cartilage Mechanics Workshop. The Combined Meeting of the Orthopedic Research
Societies of the United States, Japan and Canada. Banff, Alberta, Canada. Oct. 21st -
23rd, 1991.
Equine Osteochondrosis Workshop. First International Conference on Equine Osteochondrosis. Girton College, Cambridge, England, Sept. 25th - 27th, 1992.
Bone Morphometry Workshop. Sixth International Congress on Bone Morphometry. Lexington KY. USA. Oct. 4th - 9th, 1992.
Third Symposium on Undecalcified Bone Technology. Indianapolis IN, USA. June 8th - 10th, 1995.
Teacher-Oriented Workshops:
Teachers-on-Teaching Series Student-teacher relationships Designing instruction Techniques for using instructional technology Lecturing techniques Subjective tests and assigning grades
Problem Based Learning: Methods of Application and Learning
Diversity in the Classroom: Implications for Teaching and Learning
Using the Internet and the World Wide Web in the Classroom: Implications for Teaching
Visiting with the Queen at the Equine Osteochondrosis meetings
Surfing in Mexico with Drs. David Hankey and Gethin Thomas
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