1. Specific equipment of the facility
   • Coulter Epics V flow cytometer equipped with a 5-watt argon laser; UV excitation capabilities; High speed cell sorting; flexibility in wavelength excitation and emission detection

   • BD FACScan flow cytometer equipped with a 15mW argon laser at 488nm; three color fluorescence (510-525nm; 560-590nm; >650nm emissions); HP-based software (LYSIS program)

   • Zeiss standard epi-fluorescence microscope with various optical filters

   • Black and white high resolution video camera for fluorescence Panasonic VHS video camera recorder Black and white video monitor Microscope attachment for Zeiss epi-fluorescence microscope

   • Color video camera (not suitable for fluorescence) Panasonic VHS video camera recorder, portable 13" color monitor Microscope attachment for Zeiss microscope

   • Ethernet (thinwire) connecting:
     • - MacIntosh IIci computer system

     • - Sun 386i computer (UNIX/DOS compatible)

     • - HP300 workstation running LYSIS

2. Other equipment as part of the facility:
   • Eppendorf Microcentrifuge (model #5415)

   • IEC centrifuge Centra 4

   • Mettler AE163 balance (resolution to 0.1mg)

   • water filtration system (down to 0.2um)

   • Fume hood

   • Refridgerator; freezer for sample and/or chemical storage

   • VWR Model 2020 incubator

   • pH meter

   • variety of plankton nets

   • Submersible pumping system to obtain subsurface natural population samples

   • Coulter Counter Model ZM

3. Bigelow Laboratory equipment for general use:
   • Culture room with pre-existing light and temperature conditions

   • four Percival culture boxes (adjustable light and temperature regimes)

   • culture transfer room

4. Supplies/services available but for which user may be charged:
   • a variety of fluorescent probes

   • algal cultures purchased through the CCMP Facility

   • support staff time to prepare large volumes of culture, etc.

5. Ancillary equipment: use is dependent on other research grant commitments
   • Image cytometry systems:
     1. slow scan, cooled CCD camera (Photometrics)
     2. Sony 3-chip intergrating clor video camera
     3. Zeiss AxioScop equipped for fluorescence and phase
     4. Mac Quadra 900 for image display and analysis

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu