ISAC Specialty Training & Education Program

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Ligand - Receptor Interaction, Cell Adhesion and Whole Blood Cytometry

University of New Mexico
Cytometry Laboratory
University of New Mexico School of Medicine
900 Camino de Salud, NE
Albuquerque, New Mexico 87131-5636 

Laboratory Profile

Head: Larry A. Sklar, Ph.D.; Technical Director: Larry Seamer, BS, MT (ASCP)

1. Facilities: 3000 square foot facility containing instrumentation lab, general biology lab, computer facilities, tissue culture facility, molecular biology facility for preparation of vectors, transfection, and expression.

2. Instrumentation:
Flow cytometry: FACScan (2), Epics Elite, Epics 753.
Other major items: Hitachi spectrophotometer; SLM 8000 spectrofluorometer; SLM 4850 MHF spectrofluorometer; Applied Photophysics DX. 18 double mix spectrophotometer; Zeiss phase-contrast microscope; Meridian InSIGHT plus laser scanning, confocal microscope; Beckman elutriator centrifuge.

3. Specialization Areas:
Ligand-receptor analysis: Receptor site number; binding constants and rates; receptor internalization; computational modeling.
Whole blood cytometry: Physiology of cells in blood; adhesive interactions; receptor expression; leukocyte-platelet interactions.
Cell-cell interactions: Heterotypic and homotypic adhesion analysis; receptor expression.

4. Special Opportunities: Ligand-receptor interactions: Ligand-receptor analysis and modeling. Isolation, preparation, handling of human neutrophils. Handling of blood; assay of priming and receptor expression in patient samples.

5. Special Courses Offered: None at this time.

6. Graduate Program: Mentors associated with UNM Structural Biology Center and Cytometry Laboratory.

7. Local Accommodations: Nearby hotels and motels ranging from $25 - $75 per day. Closest is one mile. Some provide van transportation to UNM.

8. Transportation: Local airport is Albuquerque International, 4 miles from UNM; cab is approximately $6.00

9. Cost of Internship: Negotiable.

10. Communication:

Larry A Sklar, Ph.D.
Professor of Pathology
University of New Mexico School of Medicine
Cytometry/UNM Cancer Center, Room 325
900 Camino de Salud, NE
Albuquerque, New Mexico 87131-5636

Phone: (505) 277-7249
FAX: (505) 277-6677
E-mail: LSKLAR@MEDUSA.UNM.EDU 

International Society for Analytical Cytology
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If you have suggestions or comments related to the STEP Program in general, problems with this particular page, or you want to know how to be a STEP program lab, send me an email message now robinson@flowcyt.cyto.purdue.edu

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu