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Biological Dosimetry, Toxicology and Environmental Studies
GSF-Institut fur Blophysiskalische Strahlenforschung
AG-Durchflusszytometrie
Ingolstaedter Landstrasse I
D-85785 Oberschleissheim, GERMANY
Laboratory Profile
Director: Dr. Michael Nuesse
1. Facilities available: General biology laboratory, tissue culture laboratory, instrumentation laboratory, cornputer facility.
2. Instrumentation:
Flow cytometry: FACStar+ cell sorter with two lasers (360 nm-528 nm), Cytofluorograph 30 (single laser, 36C nm-514 nm), Slit-Scanning system and optical experimental setup with 2 lasers, possible use of a FACScan in separate building.
Other major items: Fluorescence microscope with color cooled CCD imaging system, fluorescence spectrometer, UV/VIS spectrometer, FTIR- and RAMAN spectrometer, various fluorescence microscopes, irradiation sources (Cs 137 , Co 60 , etc) available for radiation experiments, UV-irradiation facility (solar simulator, UVB, UBC).
Computers and Analytical: All data analysis is IBM PC-based; original software (such as LYSIS II) only used for data acquisition, an own software developnet (DAS V4.29) is used for flow analysis including slitscanning. Network: VINES Version 5.5. The co nnection to mainframe computers of a large research facility is possible by the network, communication via E-mail as well.
3. Specialization areas: Research for biological dosimetry, toxicology and general environmental aspects. Flow cytometfic analysis of micronuclei and chromosomes, cell kinetic studies with BrdUrd incorporation (BrdUrd/Hoechst or immunofluorescence). Fl ow cytometric analysis of bacteria and algae possible.
4. Training Opportunities: All aspects of experimental design, cell culture, preparation, staining, flow measurement and sorting, computer data analysis, Networking, etc.
5. Special courses offered: None
6. Graduate Program: None
7. Local Accomodations: Guest house of the GSF with limited number of rooms, approx. 30 DM per night, other hotels in Munich available from ca 100 DM.
8. Transportation: Munich International Airport ca. 30 km, Railway station Munich Center 15 km, Autobahn connection Exit Neuherberg,
9. Cost of Internships: Not indicated
10. Communication:
Dr. Michael Nuesse
GSF-Institut fur Blophysiskalische Strahlenforschung
AG-Durchflusszytometrie
Ingolstaedter Landstrasse I
D-85785 Oberschleissheim, GERMANY
Phone: (49) 89 3187 3426
FAX: (49) 89 3187 3349
E-mail: Nuesse@gsf.de
Specialty Training & Education Program
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