ISAC Specialty Training & Education Program

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Coulter Cell Sizing Technology in Flow Cytometry Systems

Max-Planck-Institut fur Biochemie
Arbeitsgruppe Zellmebtechnik
82152 Martinsried/Munchen, GERMANY

Laboratory Profile

Director: Dr. Ing. Volker Kachel

1. Facilities available: 150 m 2 including laboratory, electronic workshop, mechanical workshop.

2. Instrumentation:

Flow cytometry: Metricell Cell Volume analyzers, Fluvo-Metricell analyzers for cell volume and arc lamp based fluorescence analysis. Macro Flow Planktometers for electrical voulme, laser based fluorescence and light scatter and flow cytometric imaging of marine objects in the size range between 0.1 mm-3 mm. Experimental setup for electrical sizing of fish larvae. Experimental setup with laser based flow cytometry and flow cytometric imaging for objects in the 2mm -50 mm size range.

Computers and software: 10 IBM-PC based systems available in the group (80286-80486). All FCM data acquisition and analysis is based on CYTOMIC LIST PC hardware and software, developed in the group. Non cytometric software in use: Wordstar, Word for Wi ndows, Autocad, Corel Draw, Draft Choice, Eagle for electronic design, Mathcad.

Machine tools for mechanical work, equipment for electronics, design and test: PC based PLD design system (Data 1/0), oscilloscopes, counters pulse generators, etc.

3. Specialization areas: Development of flow cytometric systems and procedures including electrical sizing, optical analysis and flow cytometric imaging. Flow cytometric imaging means imaging of cells and organisms in flow directly in the flow cytometri c transducer and triggered by the flow cytometric analysis. Application of the systems for plankton analysis in cooperation with marine research institutions.

4. Training Opportunities: Technical and methodological aspects of electrical cell sizing combined with optical methods and flow cytometric imaging. Systematic influences on the biological material. Limitations of the methods.

5. Special courses offered: None. Cooperation on individual arrangements.

6. Graduate Program: None.

7. Local Accomodations: Local accomodation in the guest house of the Institute. Accomodation and meals about 40 DM per day.

8. Transportation: Munich International Airport, Railway connections via Munich. Local connection via the Munich public transportation systems (S-bahn, buses, tramway, underground).

9. Cost of Internships: Depends on the studies to be performed and has to be individually negotiated.

10. Communication:

Dr.Volker Kachel
AG Zellmebechnik
Max-Planck-Institut fur Biochemie
82152 Martinsried/Munchen, GERMANY

Phone: 089 8578 2571
FAX: 089 8578 3777


International Society for Analytical Cytology
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If you have suggestions or comments related to the STEP Program in general, problems with this particular page, or you want to know how to be a STEP program lab, send me an email message now robinson@flowcyt.cyto.purdue.edu

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu