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Microbiological Studies, Sorting, Immunophenotyping, Cell Function Studies
Centro de Citometria de Flujo
Universidad Complutense
Facultad de Farmacia
Ciudad Universiwia, s/n
28040 Madrid, SPAIN.
Laboratory Profile
Director: Miguel Sanchez Perez
Research Manager: Alberto M. Alvarez Barrientos
1.Facilities available: 10 m 2 facilities containing cytometer facility, microbiology and molecular biology labs, tissue culture room and office space.
2. Instrumentation:
Flow Cytometry: Becton Dickinson FACStar PLUS with Innova 70-2 argon laser and spectrum laser with UV and red emission, ACDU and pulse processors. Also available two FACScan.
Microscopy: Bio-Rad MRC 1000 inverted confocal microscope with air-cooled dual emission argon laser. Floroscence microscope (inverted and right configuration).
Other major items: Beckman spectrophotometer, ELISA plate reader. Tissue culture facilities. General molecular biology lab instrumentation.
Computers and Analytical: Data analysis software is based in Becton Dickinson CONSORT 30 and CONSORT 32programs. Included FACScan and FACStar PLUS research software, CHRONYS, CellFit, Paint-a Gate and Paint-a Gate Plus, LYSYS II, PC-LYSYS, etc.
3. Specialization areas: General Microbiology, including morphogenesis and cell integrity studies, species identification, reporter genes (b-gal and exoglucanase), metabolic patterns through cell cycle (proteins, pH. viability, etc.), yeast cell-cycle mu tants, plasmids, etc. Hepatotoxicity, viral infection kinetics, growth factor effects (pH, Ca ++ , membrane potential, redox state, etc.). Use of probes for oxidation, including DCFH-DA, Hydroethidine and dihydrorhodamine 123, Immunophenotyping. Cell sorti ng for Microbiology and general applications.
4. Training Opportunities: Microbiological applications of flow cytometry; Sorting; Use of metabolic probes; Immunophenotyping.
5. Special courses offered: FACScan and FACStar PLUS instrument usage. Data analysis and Acquisition Software courses.
6. Graduate Program: None.
7. Local Accomodations: University facilities close to the lab (about 45$ per night including breakfast).
Local hotels with a wide price range.
8. Transportation: Local airport is Madrid-Barajas airport (MAD), regularly conected with major international airports. Bus shuttles from/to the airport cost 2$. Underground from the center of town to the University.
9. Cost of Internships: Base rate of 70.000 PTA (about 550$), including all the reagents and the flow cytometer time. The best time for courses and internships is during July and August.
Alberto M. Alvarez Barrientos
Centro de Citometria de Flujo, U.C.M.
Facultad de Farmacia
Ciudad Universiwia, s/n
28040 Madrid, SPAIN.
Phone: (34) 1 394 1832
FAX: (34) 1 394 1745
E-mail: ccf@far.ucm.es.
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