 |
 |
 |
 |
[Introduction to STEP]
[Table of Contents]
[STEP HomePage]
[Search for Keyword]
DNA Analysis
Laboratorio de Citometria
Centro de Formacao e Treino em Biotecnologia Medica
Centro de Investigacao em Biopatlogia e Oncologia - CIBO
Rua Dr. Roberto Frias
P-4200 Porto, PORTUGAL
Laboratory Profile
Director: Filipe Sansonetty, MD
1.Facilities available: a) 125 m 2 flow cytometry facility containing flow instrumentation, general biochemistry lab, computer workstations facilities including access to international networks, tissue culture facility, office space for visiting scientis ts; b) Facilities available in the Institute that may be of interest to trainees: three consulting rooms where biopsies (namely fine needle aspiration biopsies) can be obtained; tumor sample registry room (samples sent by hospital) and a tumor bank, a mult imedia room, a library with access to several databases.
2. Instrumentation:
Flow Cytometry: Coulter EPICS CS with Innova 90 argon laser. A second flow analyzer with an air-cooled laser will be available in a short term.
Other major items: Spectrofluorometer; Fluorescence microscope; Optical filters; Reasonable stock of fluorophores.
Computer and Analytical: PC platforms for flow cytometry data analysis; Software includes Coulter Cytology, Phoenix, Verity, non-cytometry software (Maclntosh and PC) expertise in programs of graphics processment and statistics.
3. Specialization areas: DNA analysis; Cell cycle analysis; tumor markers; proliferation and differentiation.
4. Training Opportunities: Obtention, preparation and handling of several types of human tumor samples; Studies involving tumor cell lines for phenotypic characterization; several anti-human antibodies available for training.
5. Special courses offered: Flow instrument operation, Routine DNA analysis and reporting; Lymphocyte phenotyping; Training on computer analysis packages.
6. Graduate Program: Program offered in several areas (e.g.: Master Degree in Oncobiology) using cytometric technologies related to studies of Blopathology and Oncology. Three courses are offered for graduate students, including technicians: a) Introduc tion to Basic Principles of Flow Cytometry; b) Practical Applications of Flow Cytometry, namely of Clinical Cytometry; c) Advanced Course on Functional Cytometry.
7.Local Accomodations: Residencial Palanca Hotel (37$ per night, including breakfast) at 10 min distance by bus; Rooms may be arranged in a student Residential of Porto University. Current rate as low as 100$ per week, including breakfast.
8. Transportation: Local airport is Sa Cameiro International Airport. 15 min by taxi (about 12$).
9. Cost of Internships: Depends upon what studies are required. A base rate of 570$ per week (15 hours x 38$/hour) has been calculated only to cover most laboratory costs including basic reagents and namely the use of the cytometer. Dates for internshi p will be defined by arrangement.
10. Communication:
Filipe Sansonetty, MD
Director, Laboratorio de Citometria
Centro de Formacao e Treino em Biotecnologia Medica
Centro de Investigacao em Biopatlogia e Oncologia - CIBO
Rua Dr. Roberto Frias
P-4200 Porto, PORTUGAL
Phone: (351) 2 550 4381
FAX: (351) 2 550 39 40
E-mail: available in January 1995
|
|
|
|