Tissue Cross Match on Coulter XL
Robert Pyle (pyle@smtpgw.kfshrc.edu.sa)
Mon, 11 Nov 96 10:02:48 SAU
We have been performing tissue crossmatch for our transplant service
using the BD FACScan. We acquire our data in a logarithmic
preference. We analyze our data changing our preferences to
geometric/channel values. We calculate the 2SD value for our controls
and then consider any value above the 2SD to be positive. We acquire
in Logarithmic and analyze in geometric/ in order to detect subtle
changes.
We want to be able to utilize our Coulter XL with MCL loader but the
software does not allow such preference changes. We wonder how other
laboratories performing similar flow crossmatches with the Coulter XL
perform similar data analysis as on the FACScan.
I would appreciate some feedback on flow crossmatch data analysis on a
Coulter XL. Also, I would invite any comments on data validity
regarding the preferences chosen for data acquisition and data
analysis.
Haywood Pyle
pyle@kfshrc.edu.sa
King Faisal Specialist Hospital
Riyadh, Saudi Arabia
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
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EMAIL robinson@flowcyt.cyto.purdue.edu
