Dead Cell Marker on 633 nm FL4 ?

Matthias Haury (mhaury@pasteur.fr)
Thu, 7 Nov 1996 17:56:49 +0100

Hello me again,

Those guys out there that have already experience with the FACScalibur 4
Colors (or equivalent setup on another machine), is there a dye that you
can use on the FL4 to exclude dead cells, while still using FITC, PE,
Tricolor for Surface markers ?

I remember to have heard something about TOPRO etc, but which works the best ?

Thanks for details (dye, manufacturer, compensations, staining concentrations).

Merci,

Matthias

______________________________________________________________
Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry - Immunology _/ _/ _/ _/ _/
Institut Pasteur Paris _/ _/_/_/ _/_/_/
Email: mhaury@pasteur.fr _/ _/ _/
Tel: 33 (01) 40 61 31 29 _/_/_/ _/ _/
Fax: 33 (01) 45 68 86 39 INSTITUT PASTEUR PARIS
______________________________________________________________

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