Tunel in skeletal muscle
tarnok@medizin.uni-leipzig.de
Thu, 7 Nov 1996 18:11:51 +0000
Dear colleagues,
our group is interested in investigating the apoptotic process in
biopsy samples of human skeletal muscle (formalin-fixed,
paraffin-embedded). So far we tried the TUNEL method with kits from
Oncor and Boehringer. Following the protocol of the company we
received the following results; our negative controls (no TdT) stayed
negative as expected, our positive control section (human tonsil
tissue) looked good. However, using the skeletal muscle biopsy
specimens we stained all nuclei positive. Also changing the blocking
conditions and the dilution of the flourescin antibody we received no
change. Our question now is if somebody has experience with TUNEL on
skeletal muscle samples, or if somebody observed the same strange
result. Thank you very much for your help
Volker Adams, PhD
Heart-Center Leipzig
Germany
***************************************************************
Dr. Attila Tarnok phone:**49-3418652430
Clinics for Pediatric Cardiology fax: **49-3418651405
Heart Center Leipzig GmbH
University Hospital, Russenstr.19
D-04289 Leipzig, Germany
Internet:tarnok@server3.medizin.uni-leipzig.de
***************************************************************
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
